Fig. 2. Results of DLS (black) and NMR (blue) measurements of nanoparticles prepared with different concentration of surfactant (A) or PLGA (B). (A) Influence of PVA concentration on particle radius and PFCE encapsulation. Particles were prepared using following concentrations of surfactant: 0.4 wt%, 1.2 wt% and 2 wt%. Here, the concentration of polymer in organic phase was the same for all samples, 1.75 wt%. Better reproducibility of particle radius was achieved with higher concentration of surfactant. Furthermore, higher concentration of PVA resulted in nanoparticles with higher encapsulation efficiency of PFCE. Error bars represent the standard deviation (n = 3) (B) influence of concentration of PLGA on particle radius and PFCE encapsulation. Higher concentration of polymer resulted in slightly larger particles, but lower encapsulation efficiency. For all samples concentration of surfactant at 2 wt% was used. DLS results are presented in black and NMR results in blue. DLS: c(NP) = 0.1 mg ml⁻¹, NMR with TFA as internal reference.