Fig. 5. Comparison of invasion-related MMPs expression between different culture conditions. Protein expression of MMP-9 and MMP-10 for OV-NC and OV-206 cells in the 2D and 3D cultures was detected by IHC staining (A) and western blotting (B). Gene levels of MMPs in the two OvCa cell lines in different cultures were examined by qRT-PCR (C). Expression data was normalized against β-actin. Each bar represents the mean ± SD. *P < 0.05. n = 3 independent experiments.