. 2018 Feb 28;8(16):8910–8919. doi: 10.1039/c7ra13742g

The primer sequences for qRT-PCR analysis.

Genes	Primer sequences	T _m (°C)
mmp-1	F: AATTTGCCGACAGAGATGAAGTC	48.4
mmp-1	R: TCAGAAAGAGCAGCATCGATATG	48.4
mmp-2	F: TGATGGTGTCTGCTGGAAAG	46.6
mmp-2	R: AGCAAACCTCGAACAGATGC	46.6
mmp-3	F: TGATGGACCTGGAAATGTTTTG	46
mmp-3	R: AGGGAGTGGCCAATTTCATG	46
mmp-9	F: TCTTCCCCTTCACTTTCCTG	43.8
mmp-9	R: TGTCGCTGTCAAAGTTCGAG	43.8
mmp-10	F: GAAGATGCATCAGGCACCAA	46.6
mmp-10	R: ATCTTGCGAAAGGCGGAACT	46.6
mmp-13	F: GGACCCTGGAGCACTCATGT	45.3
mmp-13	R: CTTGCCCTCTACAACCAACA	45.3
TGF-β1	F: CATTTGTCTGGCGTTTTTGGA	48.7
TGF-β1	R: CTTGCGACCCACGTAGTAGA	48.7
Smad4	F: GCATTCCAGCCTCCCATTT	51.2
Smad4	R: TCCAGAAGGGTCCACGTATCC	51.2
Wnt5b	F: CGCCAATGATGAACATCTC	47.8
Wnt5b	R: ATTGGGATGGGTTGAGGC	47.8
β-catenin	F: AAGGTGTGGCGACATATGCA	50.2
β-catenin	R: CAAGTCCAAGATCAGCAGTCTCA	50.2
Snail	F: CTGCGTGGGTTTTTGTATCC	46.6
Snail	R: TCTGTCAGCCTTTGTCCTGTAG	46.6
Slug	F: AGCCTTTTTCTTGCCCTCACT	49.7
Slug	R: GGACTCACTCGCCCCAAAG	49.7
Fibronectin	F: CCTGGAGCAAGAAGGATAATCG	49.7
Fibronectin	R: GGTAAACAGCTGCACGAACATC	49.7
Vimentin	F: GAAATTGCAGGAGGAGATGC	46.6
Vimentin	R: CGCATTGTCAACATCCTGTC	46.6
E-cadherin	F: CTGACACACCCCCTGTTGGT	47.3
E-cadherin	R: CCATTGGATCCTCAACTGCAT	47.3
N-cadherin	F:TAGCTAATCTAACTGTGACCGATAAGG	53.4
N-cadherin	R: TTGGTTTGACCACGGTGACTAA	53.4
Integrin α5	F: ATGCTCCATGTAGATCACAAGAT	55.7
Integrin α5	R:TTCCCAAAGTCCTTGCTGCT	55.7
Integrin β1	F: CTTGCCCTCTACAACCAACA	53.4
Integrin β1	R: TGCCCCGGTACGTGATATTG	53.4
β-actin	F: GGACTATGACTTAGTTGCGTTAC	48.4
β-actin	R: TTTGCATTACATAATTTACACGA	48.4