Fig. 2. Two-temperature PCR experiments with the polypropylene microfluidic chip. (A) Schematic of the PCR experimental device. The polypropylene microfluidic chip was placed on a heating plate with 3 heating regions (90 °C–60 °C–90 °C) to perform 20 cycles of PCR reaction. (B) The profile of the PCR experimental facility. The heat generated by the copper heating film was rapidly conducted to the solution in the microchannel through the 20 μm thick polypropylene film (cover film). The temperature sensors mounted on the bottom surface of the copper heating film.
