| pcDNA-linc00472 |
|
BamHI and XhoI |
| Forward |
CGGGATCCATGCGAGGCTGGGGCCGGTTG |
|
| Reverse |
CCGCTCGAGTTTAGACTCCAAATGGCTG |
|
| pmirGLO-linc00472-WT |
|
SacI and XhoI |
| Forward |
CGAGCTCACCCTCTACCTTTCCAATC |
|
| Reverse |
CCGCTCGAGGTTACCTATCACTTCCTTTCG |
|
| pmirGLO-PDCD4-WT |
|
SacI and XhoI |
| Forward |
CGAGCTCATATAAGAACTCTTGCAGTC |
|
| Reverse |
CCGCTCGAGACCAGGTTCATTTTTCC |
|
| pLenti-puro-linc00472 |
|
BamHI and EcoRV |
| Forward |
CGCGGATCCATGCGAGGCTGGGGCCGGTTG |
|
| Reverse |
GCGCGATATCTTTAGACTCCAAATGGCTG |
|
| pLenti-puro-pre-miR-141 |
|
BamHI and EcoRV |
| Forward |
CGGGATCCGGGTCCATCTTCCAGTAC |
|
| Reverse |
GCGCGATATCGAGCCATCTTTACCAGAC |
|
| miR-141 (reverse transcription primer), linc00472 (quantification primers) |
GTCGTATCCAGTGCAGGGTCCGAGTATTCGCACTGGATACGACGCTGA |
|
| Forward |
CACTGGGCATTTTCTCTTCA |
|
| Reverse |
CCTATCCCTTTCCCTCTGCT |
|
| PDCD4 (quantification primers) |
|
|
| Forward |
GGCCTCCAAGGAGTAAGACC |
|
| Reverse |
AGGGGTCTACATGGCAACTG |
|
| β-Actin (quantification primers) |
|
|
| Forward |
GCATCCCCCAAAGTTCACAA |
|
| Reverse |
AGGACTGGGCCATTCTCCTT |
|
| miR-141 (quantification primers) |
|
|
| Forward |
GCCGCTGTAAACATCCTACACT |
|
| Reverse |
GTGCAGGGTCCGAGGT |
|
| U6 snRNA (quantification primers) |
|
|
| Forward |
CTCGCTTCGGCAGCACA |
|
| Reverse |
AACGCTTCACGAATTTGCGT |
|
