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Fig. 2. Percentage changes in the three dominant fatty acids (>10% each of the total fatty acid content) determined as a percentage of total membrane content; shown as averages ± standard deviations of five biological replicates of E. faecalis OG1RF wild type, EFC3C and EFC3Py. Significant differences between the mutants EFC3C and EFC3Py compared to the wild type was calculated using two-way ANOVA and Tukey's multiple comparisons test and corrected p-values indicated with *p ≤ 0.0001.