Minimum inhibitory concentrations of COE1-3C, COE1-3Py and daptomycin.
| Strain | Description | Growth conditions | MIC | ||
|---|---|---|---|---|---|
| COE1-3C | COE1-3Py | Daptomycin | |||
| E. faecalis OG1RF | Wild type | BHI | 2 μM | 1 μM | 1.23 μM |
| EFC3Ca | COE1-3C resistant | BHI | 8 μM | 1 μM | 9.87 μM |
| EFC3Pyb | COE1-3Py resistant | BHI | 8 μM | 16 μM | 9.87 μM |
| DAP 21c | Daptomycin resistant | BHI | 2 μM | 1 μM | 78.96 μM |
| DAP 22d | Daptomycin resistant | BHI | 2 μM | 1 μM | 78.96 μM |
| E. faecalis OG1RF | Wild type | BHI + palmitic acid C16:0 (10 μg mL−1) | 2 μM | 1 μM | ND |
| E. faecalis OG1RF | Wild type | BHI + cis-vaccenic acid C18:1 ω7c (10 μg mL−1) | 8 μM | 2 μM | ND |
| E. faecalis OG1RF | Wild type | BHI + cis-9,10-methyleneoctadecanoic acid C19:0 cyclo ω8c (12.5 μg mL−1) | 8 μM | 4 μM | ND |
COE1-3C resistant strains with in frame deletion in liaF at position 179 and a substitution in the intergenic region between treB (PTS family trehalose porter, IIBC component) and gloA6 (lactoylglutathione lyase).
COE1-3Py resistant strains with a non-synonymous substitution at position 97 in the liaR gene.
DAP21 strains have mutations in cardiolipin synthase 1 (cls1; RF10364), a gene encoding a putative chaperone protein regulated by the liaFSR operon (RF11464) and hypothetical membrane protein (RF11507).21
DAP22 strains have mutations in gene encoding a putative chaperone protein regulated by the liaFSR operon (RF11464) and hypothetical membrane protein (RF11507) along with mutations in cardiolipin synthase 2 (cls2; RF11324) and a putative metal-dependent HD-domain-containing hydrolase (RF11901).21 ND – not determined.