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. 2022 Apr 30;2022:8317466. doi: 10.1155/2022/8317466

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Copyright © 2022 Zhichao Wu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of LDH reversed a portion of glycolysis in propofol-treated CRC cells. HCT-116 and LoVo cells were treated with propofol or/and LDH-overexpression plasmids. (a) An ATP assay kit was used to evaluate ATP levels in each group of cells. (b) A lactate assay kit was used to monitor changes in lactic acid levels. (c) Changes in glucose consumption were detected with a glucose assay kit. (d) The levels of HK2, PKM2, and LDHA proteins were detected by western blotting. (e) The levels of 8-OHdG were analyzed using an 8-OHdG assay kit.