Fig. 6. Antigen persistence and depot at the injection sites. Nude mice (n = 3) were injected subcutaneously (left hind leg) or intramuscularly (right hind leg) with 100 μL of the different formulations of the nanoemulsion adjuvant vaccines containing 1 mg mL−1 GFP protein. Antigen persistence at the injection sites was measured using a Carestream FX PRO in vivo imaging system. (A) Representative fluorescence images and (B and C) quantitative fluorescence intensity of the antigen persisting at the injection sites. Data are expressed as the mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
