Fig. 7. Protective effects of three vaccine antigen attachment ways in a MRSA sepsis model. Balb/c mice (n = 6) were immunised as previously described. At days 21, mice were infected with 2.5 × 108 CFUs MRSA 252. At 1 and 3 days post-infection, the bacterial burdens in the blood (A, D), spleen (B, E) and kidneys (C, F) were measured. (G) At 3 days post-infection, the kidneys were collected and the representative histopathological sections are shown (magnification = ×400). Arrowheads indicate Staphylococcal abscesses. (H) Severity scores of the kidneys (n = 6) from the six immunised groups at 3 days post-infection are shown. Data are presented as scatter plots and the means ± SD are shown. (I) The mice (n = 10) were intravenously infected with MRSA 252 (1 × 109 CFUs) and the survival rates were monitored for 14 days. *P < 0.05; **P < 0.01; ***P < 0.001.
