Primer sequences.
Gene | Product size (bp) | Primer | 5′ primer sequences 3′ |
---|---|---|---|
GAPDH | 185 | Forward | GGTGGTCTCCTCTGACTTCAACA |
Reverse | GTTGCTGTAGCCAAATTCGTTGC | ||
SOD | 115 | Forward | TGGAGATAATACAGCAGGCT |
Reverse | AGTCACATTGCCCAAGTCTC | ||
CAT | 97 | Forward | CCTTCGACCCAAGCAA |
Reverse | CGATGGCGGTGAGTGT | ||
GSH-Px | 172 | Forward | AGAAGTGCGAGGTGAACGGT |
Reverse | CCCACCAGGAACTTCTCAAA | ||
Nrf2 | 172 | Forward | AGTGTGGAGAGGTATGAGCC |
Reverse | CGTTCCTCTCTGGGTAGTAA | ||
Keap1 | 126 | Forward | AGAGCGGGATGAGTGGCA |
Reverse | GCTGAATTAAGGCGGTTTGTC |