Fig. 5.

Specimen analyses. a) Malignant lymph nodes (LN)s show over six times increased mean fluorescence intensity compared to benign LNs (p < 0.0001). b) Representative macroscopic fluorescence images of a malignant and a benign LNs from a subject diagnosed with metastases upon final histopathology. The malignant LN shows increased fluorescence signal in the tumor tissue except in areas of necrosis. This cetuximab-800CW fluorescence largely co-localizes with EGFR-expression. Earlier, we showed the tumor-specificity of cetuximab-800CW fluorescence in LNs [48]. Abbreviations: LN+ , malignant lymph node; LN-, benign lymph node; H&E, hematoxylin and eosin; EGFR, epidermal growth factor receptor.