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. 2022 Mar 2;29(24):35518–35541. doi: 10.1007/s11356-022-19423-4

Table 2.

Principle involved, along with various sources, compounds extracted, solvents used, and the corresponding process conditions used in Soxhlet extraction

Principle Sources Compounds extracted Process conditions References
Heating, condensation, extraction, and reflux of S within a Soxhlet apparatus Spent coffee (silverskin) Caffeine Hexane, dichloromethane, ethanol, 1:50, 6 h, the temperature was a solvent boiling point Mussatto et al., 2015
Chlorogenic acid

60% isopropanol (60%), 1:10,

27 °C

Waste Punica granatum L. (pomegranate) seeds Oils (PUFAs, punicic acid) Hexane, 1:15, 4 h, 60 °C Talekar et al., 2018
Rosmarinus officinalis L. (rosemary) leaves Rosmarinic acid, Carnosic acid, Carnosol

96% food grade ethanol, demineralized water, 1:12,

8 h

Hirondart et al. 2020
Piper betle (betel) leaves Eugenol, Eugenol acetate

100% acetone, 1:5, 56 °C,

8 h

Das et al., 2019
Agaricus bisporus L Ergosterol Hexane, ethanol, or limonene (150 mL for 4.5 g of sample), 4 h, Heleno et al., 2016
Anthemis cotula L. (stinking chamomile) Anthecotuloid, Caffeoyl Quinic acid, and Quercetin 96% ethanol, 1:20, 6 h Sut et al, 2019
Allium cepa L. var. ascalonicum Alkaloids, Polyphenols, Tannins, Flavonoids

70% ethanol + HCl (2 N),1:10,

pH: 1, 2 h

Saptarini and Wardati, 2020
Silybum marianum L. Gaertner fruits Silymarin n-hexane (defatting), methanol (for Silymarin extraction), 2:75, 6 h (defatting) + 5 h (actual extraction) Wianowska and Wiśniewski, 2015
Miscanthus sinensis (runo) stem Runo dye 50% ethanol and 4 h Pinzon et al., 2020