REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Xpress	Life Technologies	Cat#46-0528; RRID: AB_2556552
Alexa Fluor 488 conjugated goat anti mouse	Invitrogen	Cat#A11001; RRID: AB_2534069
Alexa Fluor 633 conjugated goat anti mouse	Invitrogen	Cat # A-21052; RRID: AB_2535719
Bacterial and virus strains
E. coli DL21 DE3 pLysS	Bio-Rad	Cat#1563003
Chemicals, peptides, and recombinant proteins
PBS 10×	Sigma-Aldrich	Cat# MFCD00131855
Goat serum	Sigma-Aldrich	Cat#G9023
DMEM	Sigma-Aldrich	Cat#D5671
FBS-12A	Quimigen	Cat# FBS-12A
Glutamax	Biowest	Cat#x0551
Trypsin solution	Thermo Fisher Scientific	Cat#25200072
Penicillin-Streptomycin	Sigma-Aldrich	Cat#P0781
L-Lysine solution	Sigma-Aldrich	Cat#P4707
Hoechst 33342	Sigma-Aldrich	Cat#14530
Immersion oil	Leica Microsystems	Cat#195371-10-9
Fluoromount-G	SouthernBiotech	Cat#0100-01
Benzonase	Merck	Cat#103773
Protease inhibitors	Thermo Fisher Scientific	Cat#A32955
Critical commercial assays
In-Fusion HD Cloning kit	Takara Bio	Cat#638920
Cell lines
HEK293T	ATCC authenticated	N/A
Oligonucleotides
5′GATAAGGATCGATGGGGATCCA TGGAAGGACGTTTCCGTC	Integrated DNA Technologies	Forward
5′GCAGATCTCGAGCTCGGATC CTTAAGCGGCGATTTC	Integrated DNA Technologies	Reverse
Software and algorithms
Fiji ImageJ 1.53c	https://imagej.nih.gov/ij/	Schindelin et al. (2012)
Other
HisTrap HP affinity column	Cytiva	Cat#17524802
ÄKTA HITrapTM 5 mL desalting column	Cytiva	Cat#17140801
pRSET-A	Thermo Fisher Scientific	Cat#V35120
Leica SP8 confocal microscope	Leica Microsystems	N/A
Round 12 mm coverslips	Thermo Scientific	Cat#15820692