Fig. 4.

MAM/SRT did not affect cell proliferation and viability. A, Calcein/PI staining analysis for BMSCs viability. Green fluorescence represents live cells. Red fluorescence represents dead cells. B, EdU fluorescence staining for BMMs proliferation. Green fluorescence represents proliferating cells. Blue fluorescence represents cellular nucleus. C, the quantitive analysis of EdU fluorescence staining. Sham, cells were treated with 0.1% DMSO using transwell insert. MAM, cells were treated with 1 mg MAM using transwell insert. MAM/SRT group, cells were treated with 1 mg MAM/SRT (10 mg/100 μg/mL) using transwell insert; ns represents no significant difference. D, CCK-8 assay for BMSCs viability. Data are expressed as the mean ± standard deviation (SD) (n = 3); ns represents no significant difference.