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MetaPhor electrophoresis of PCR-RFLP products specific for F1 and F2 fragments showing size allelic variants of wild and mutant types at codons corresponding to pvdhfr residues 33, 57, 58, 117, and 173. The restriction sites of SacII, XmnI, PvuII, StyI, and AluI are indicated. F, phenylalanine; I, isoleucine; L, leucine; N, asparagine; P, proline; S, serine; R, arginine.
