Table 3.

Microbial recovery (CFU/plate) from the hatching environment at DOE20 (∼20%, ∼50%, or ∼80% hatch) or at DOH immediately prior to hatch pull (Exp 1-3).

		Gram-negative bacteria				Staphylococcus aureus				Enterococcus spp.				Total aerobic bacteria				Aspergillus fumigatus
Exp	Trt1	20%2	50%	80%	DOH	20%	50%	80%	DOH	20%	50%	80%	DOH	20%	50%	80%	DOH	20%	50%	80%	DOH
Exp 1	NC	1	39	15	37	3	24	18	24	9	18	16	32	18	78	48	74	3	3	2	2
	EH	1	24	43	54	2	158	173	149	56	107	128	291	50	294	542	424	2	3	3	2
	PM	43	35	85	46	25	126	90	96	132	121	455	351	124	270	415	431	25	56	66	100
Exp 2	NC	0	0	0	1	74	0	0	0	0	1	0	6	3	3	2	6	14	5	4	1
	EH	4	209	13	38	13	24	64	22	22	38	166	229	79	89	268	318	13	13	9	36
	PM	18	454	211	495	17	5	18	47	8	37	51	151	15	112	196	154	17	30	39	14
Exp 3	NC	0	0	0	1	1	0	0	0	0	0	0	0	0	0	5	4	1	0	1	0
	EH	17	38	68	548	0	1	2	25	1	9	20	105	8	20	23	62	0	1	1	1
	PM	184	300	784	239	15	50	43	133	46	68	200	117	43	172	467	159	15	24	22	8

n = 3 replicate agar plates/media were exposed to the hatch cabinet environment for 1 m or 5 m based on the type of media.

Non-mannitol fermenting Staphylococcus spp. data not shown.

Darker shaded areas are related to higher CFU recovery for ease of interpretation.

¹NC: negative control; EH: egg homogenate; PM: pathogen mix.

²CFU reported for ∼20% hatch, ∼50% hatch, ∼80% hatch, or immediately prior to hatch pull at DOH as an average of three replicate plates for each time point. n = 3 replicate hatchers/treatment.