Figure 1.

Identification of novel substrates for known ribosomal protein N-acetyltransferases. 2D protein gel electrophoresis of total proteome from the WT strain labeled by Cy5 and Cy2-labeled proteome from ΔrimI strain. Horizontal axis corresponds to isoelectrofocusing, whereas vertical axis to separation by molecular mass (scale shown). Yellow spots correspond to the proteins with unchanged expression level. Red spots reflect proteins underrepresented in ΔrimI strain. Green spots correspond to proteins overrepresented in ΔrimI strain. Circled are the spots corresponding to EF-Tu—the new substrate of RimI. EF-Tu, elongation factor Tu.