Fig. 1. (A) In silico binding efficacy and toxicity profiling of BBAP-1: BBAP-1 was docked with PHD-2 protein (PDB id-2G19). Toxicity profile of BBAP-1 was estimated with TEST. (B) Effect of BBAP-1 upon MTT assay: the histogram revealed the effect of BBAP-1 on ER + MCF-7 cells by MTT assay. The cell growth was compared with the standard (tamoxifen). BBAP-1 displayed substantial cytotoxicity against ER + MCF-7 cells. Data is expressed as mean ± SD. The comparisons are made on the basis of one-way ANOVA followed by Bonferroni multiple test. *p < 0.05, **p < 0.01, ***p < 0.001 is considered statistically significant. Experiment was performed in triplicate. (C) Effect of BBAP-1 and cobalt upon in vitro PHD-2 activity: BBAP-1 demonstrates the in vitro activation of PHD-2. Cobalt was used as a negative control. The results are represented using control value as 100% PHD-2 activity. Values are represented as mean ± SD. Experiment was performed in triplicate.