Fig. 7. Effect of BBAP-1 treatment on mitochondrial mediated apoptosis: the protein was extracted from individual groups [1: control; 2: toxic control; 3: BBAP-1 + MNU (61.55 μg kg−1 s.c. + 8 mg kg−1 i.v.); 4: BBAP-1 + MNU (123.11 μg kg−1, s.c. + 8 mg kg−1 i.v.)] and subjected to immunoblotting of anti-apoptotic (Bcl-2 and Bcl-xl) and pro-apoptotic (BAX) proteins with downstream apoptotic markers (VDAC, cytochrome-c, Apaf-1 and procaspase 9) of mitochondrial mediated apoptotic pathway. The respective phenotypes for the different proteins were also validated using qRT-PCR. β-actin was used as internal loading control. The experiment was performed in triplicate. Values are presented as mean ± SD. Comparisons are made on the basis of one-way ANOVA followed by Bonferroni multiple test. All groups are compared to the toxic control group (*p < 0.05, **p < 0.01, ***p < 0.001).
