Kinetic parameters for Ylehd under steady-state conditions^a.

Compounds	Binding energy from docking studies (kJ mol−1)	k 303 K (μM−1 s−1)	K m (mM)	k cat b (s−1)	k cat/Km (mM−1 s−1)	E c
rac-PGE	−25.73	17.3 + 0.82	0.6 ± 0.009	7.34 ± 0.06	12.2 ± 0.78	1.5
(R)-PGE	−26.27	28.11 + 0.73	1.32 ± 0.045	33.79 ± 0.87	25.5 ± 0.97
(S)-PGE	−25.77	21.9 + 1.05	0.93 ± 0.071	15.66 ± 0.49	16.6 ± 0.057
rac-BGE	−26.36	21.3 + 0.08	0.15 ± 0.002	5.73 ± 0.54	38.2 ± 1.08	10.37
(R)-BGE	−27.15	18.2 + 0.47	0.8 ± 0.01	3.45 ± 0.089	4.31 ± 0.07
(S)-BGE	−28.41	31.1 + 0.97	0.27 ± 0.024	12 ± 0.97	44.7 ± 0.87

^aKinetic constants calculated by non-linear regression analysis in Microcal Origin.

^bKinetic studies for all compounds were carried out with 2 μg protein except for (R)-BGE wherein 10 μg protein was used.

^cEnantiomeric ratio (E) was calculated as ratio of k_cat/K_m of the fast-acting enantiomer over the slow acting one.