Fig. 4.

USPIO MRI shows increased blood volume after PEGPH20 treatment. MRI using USPIO was performed in BxPC3-HAS3 tumor bearing mice treated with control buffer or PEGPH20 (n = 3 each group) before (before the first injection on day 0) and after treatment (3 h after the 2nd PEGPH20 injection on day3). A, Representative imaging of USPIO intensity with T2-weighted anatomical image. Center four slices of each tumor are shown. B, Blood volume (%) of region of interest in each tumor (blue line in Fig. 3A) before and after treatment are shown by treatment group. Individual values are shown. C, Blood volume change (%) from the baseline (pre-treatment) of each group. Data are shown as mean ± SE. Statistical significance between groups was determined by paired t-test for B and by Student's t-test for C.