FIG. 3.

The antifungal activity of rapamycin and its analogs is mediated via FKBP12 and TOR proteins. C. albicans (A), C. neoformans (B), and S. cerevisiae (C) wild-type and FKBP12 (rbp1/rbp1, frr1, or fpr1) and TOR (TOR1-1/TOR1, TOR1-1, and TOR2-1) mutant strains were grown in YPD liquid medium overnight, and ∼10⁶ cells were resuspended in the top agar on the surface of YPD medium. Disks without (−) or with 1 μg of rapamycin (R) or analogs 2, 18, 19, and 23 were placed on the surface of the medium and incubated for 24 to 36 h at 30°C.