Fig. 3. Effects of reaction conditions on the fluorescent signals and F₁/F₀ values of the proposed method. (a) Concentration of strand a (strand b, c, d and e: 100 nM; EpCAM: 50 ng mL⁻¹). (b) Concentration of strand d (aptamer/strand a: 30 nM; strand b, c, d and e: 100 nM; EpCAM: 50 ng mL⁻¹). (c) Concentration of strand e (aptamer/strand a: 30 nM; strand b, c, and d: 100 nM; EpCAM: 50 ng mL⁻¹). (d) The reaction time (aptamer/strand a: 30 nM; strand b, c, d and e: 100 nM; EpCAM: 50 ng mL⁻¹).