Fig. 6. SNHG3 knockdown suppressed breast cancer tumor growth and invasion in vivo by upregulating miR-101 and downregulating ZEB1. Lenti-anti-miR-con or lenti-anti-miR-101 was transduced in lenti-sh-SNHG3 or lenti-sh-con stably transfected MCF-7 cells and then MCF-7 cells were subcutaneously injected into nude mice. (A) Tumor volume was measured every 7 days for 42 days. (B) Forty-two days after injection, the mice were sacrificed and the tumor tissues were isolated and weighed. (C) The expressions of SNHG3 and miR-101 in xenografted tumors. (D) The protein levels of ZEB1, Ki-67, PCNA, MMP-2 and MMP-9 in xenografted tumors were detected using western blot analysis. *P < 0.05.