TABLE 2.
Effects of filtration and washing on the reversibility of the putative MurA-inhibitor complex
| Compound | % Inhibitiona
|
Reversible | |
|---|---|---|---|
| Before filtration | After washing | ||
| Fosfomycin | 111 ± 10 | 132 ± 10 | No |
| RWJ-3981 | 116 ± 17 | 101 ± 3 | No |
| RWJ-110192 | 101 ± 13 | 36 ± 24 | Yes |
| RWJ-140998 | 77 ± 27 | 102 ± 10 | No |
a
Average of two (RWJ-3981) or three experiments. MurA and each inhibitor were subjected to three cycles of ultrafiltration and dilution to remove free inhibitor and then assayed for activity as described in Materials and Methods.