Fig. 1. Effects of different treatments on proliferation and differentiation abilities of C2C12 myoblast cells. (A) Cell morphologies after being fixed in 4% paraformaldehyde (scale bar: 100 μm). (B) Cell numbers corresponding to panel (A). (C) MTS cell proliferation assay. (D) Western blot analysis showing the expression of MyoD1. The anti-actin antibody was used to standardize the amount of protein in each lane. (E) Statistical analysis corresponding to panel (D). *P < 0.05, **P < 0.01, ***P < 0.001.