Fig. 5. Keratinocyte–hMSC co-cultivation on the 1% sericin loaded Bio-Syn composite scaffold. (A) Four quadrant gated FACS analysis of keratinocyte (CTB)–hMSC (CTG) contact co-cultured. (B) Keratin 14 and Np63α gene-expression analysis was assessed using qRT-PCR with mRNA transcript in a co-culture model of keratinocyte–hMSCs. hMSCs cultivated alone on the respective scaffold were used as the control. (C) A hMSC sub-population acquired a round keratinocyte-like morphology (red arrow), whereas another sub-population became stretched and attained a fibroblast-like shape (yellow arrow), liberated from the Bio-Syn composite scaffold type. During co-culture, multinucleated cells (red circle) were also witnessed. hMSCs cultivated on 10% Bio-Syn composite scaffolds were considered as the control. Data are represented here in terms of the mean ± SD. (n = 3).