Evaluation of biochemical parameters and pro-healing markers in the Bio-Syn composite scaffold, commercial dressing (Neuskin™) and cotton gauze control treated groups. Data are presented as mean ± SD; n = 6 (for Neuskin™: *p < 0.05, **p < 0.01, ***p < 0.001) (for cotton gauze: #p < 0.05, ##p < 0.01, ###p < 0.001) (mg g−1 of dry tissue weight)/(mg of dry tissue weight). (+++) represents the maximum response while (−) refers to the minimum response.
| Parameter | Bio-Syn composite scaffold | Cotton gauze control | Neuskin™ | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Day 7 | Day 14 | Day 21 | Day 7 | Day 14 | Day 21 | Day 7 | Day 14 | Day 21 | |
| DNA (mg g−1) | 2.8 ± 0.1## | 4.02 ± 0.3# | 4.35 ± 0.2## | 1.7 ± 0.37 | 2.56 ± 0.4 | 3.95 ± 0.5 | 2.1 ± 0.6 | 3.68 ± 0.2 | 4.25 ± 0.4 |
| Protein (mg g−1) | 133 ± 2.1### | 152 ± 3.7### | 165 ± 4.2 | 92.2 ± 1.36 | 137 ± 3 | 156 ± 5.6 | 129.6 ± 2.3 | 146 ± 1.5 | 158 ± 1.7 |
| HAE (mg g−1) | 0.9 ± 0.02***,### | 1.1 ± 0.05**,### | 1.14 ± 0.03# | 0.53 ± 0.07 | 0.89 ± 0.04 | 1.05 ± 0.04 | 0.59 ± 0.02 | 0.96 ± 0.04 | 1.09 ± 0.08 |
| HP (mg g−1) | 47 ± 3.23**,## | 62 ± 4.93*,# | 84 ± 3.7# | 32.9 ± 3.03 | 49 ± 4.21 | 77 ± 2.1 | 35.07 ± 3.6 | 47 ± 5.1 | 78 ± 1.8 |
| Acute inflammation | ± | ± | − | +++ | ++ | ± | + | ++ | − |
| Chronic inflammation | ± | ± | − | ++ | + | ± | + | + | − |
| Edema | + | − | − | +++ | + | + | + | + | − |
| Granular tissue formation | ++ | +++ | +++ | ++ | + | + | ++ | + | + |
| Collagen formation | ++ | +++ | ++ | + | ++ | + | + | ++ | ++ |
| Re-epithelization | ++ | +++ | +++ | ± | + | ++ | − | + | + |