Fig. 6. Analysis of the chondrogenic capacity of hMSCs cultured on CTS and CC hydrogel. (A) mRNA transcript levels of cartilage-related markers of hMSCs were analyzed using quantitative RT-PCR. (B) Western Blot analysis of chondrogenesis proteins after hMSCs was cultured for 21 days. (C and D) Histologic analysis of hMSCs cultured on CTS and CC hydrogel for 21 days. Specimen sections were stained with (C) H & E or (D) Alcian Blue. Scale bar: 50 μm. (E) GAG production in hMSCs cultured with CTS or CC hydrogels was quantified and normalized with the total DNA amount. **p < 0.01, n = 3.