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| Gene name | Accession number | Primer sequences (5′ to 3′) |
|---|---|---|
| GAPDH | NM_002046.3 | F: CTCTCTGCTCCTCCTGTTCG |
| R: TTAAAAGCAGCCCTGGTGAC | ||
| SOX9 | NM_000346.3 | F: TAAAGGCAACTCGTACCCAA |
| R: ATTCTCCATCATCCTCCACG | ||
| COL2 | NM_001844.4 | F: CCTCTGCGACGACATAATCT |
| R: CTCCTTTCTGTCCCTTTGGT | ||
| AGN | NM_013227.2 | F: CACGATGCCTTTCACCACGAC |
| R: TGCGGGTCAACAGTGCCTATC |
Forward and reverse primers are indicated as “F” and “R”, respectively.
