Fig. 5. Hydrolytic cleavage of complexes (1–6) on pBR322 DNA (33.3 μM) in Tris–HCl/NaCl buffer. Lane 1, DNA alone, lane 2–7, DNA + 1–6 (25 μM) (a). Analysis of the capacity of T₄ DNA ligase to relegate DNA cleaved by complexes 1–6. Lane 1–6, NC obtained using complexes 1–6 + T4 ligase (b).