Fig. 1. TALEN design and OGDH^−/− rat establishment. (A) Shows the assembly of TALEN. (B) Shows the detection principle of TALEN activity. The red mark represents a terminator, and the blue marker represents a TALEN target position sequence. The target location produces DSB under the action of TALEN. Then, cells repair DNA by homologous recombination and form an active luciferase. (C) Shows the activity level of TALEN by the change of reference ratio. (D) Shows the OGDH gene sequencing result of WT rats. (E) and (F) show two different situations of the OGDH sequencing result of the OGDH^+/− rats. The red bases in (G) show the TALEN targets in WT rats, and these bases are missing in the OGDH^+/− rats.