Reactivation of latent HIV-1 gene expression by the indicated quinolines in J-Lat A2 cellsa.
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|---|---|---|---|---|---|---|---|---|
| Compound | n | R5 | % GFP(+) cells (μM) | % Cell viability (μM) | ||||
| 10 | 5 | 1 | 10 | 5 | 1 | |||
| DMSO | 0.7 ± 0.1 | 0.7 ± 0.2 | 0.7 ± 0.2 | 98.6 ± 0.5 | 98.1 ± 1.9 | 98.8 ± 0.6 | ||
| SAHA | 71.2 ± 4.2 | 56.8 ± 1.2 | 20.1 ± 0.3 | 43.2 ± 3.2 | 50.9 ± 5.1 | 80.4 ± 2.4 | ||
| AV6/7a | 1 | H | 57.0 ± 1.2 | 3.8 ± 1.1 | 0.5 ± 0.5 | 47.6 ± 5.6 | 73.4 ± 3.5 | 80.7 ± 5.4 |
| 10a | 3 | COOC2H5 | 3.3 ± 0.2 | 3.2 ± 0.5 | 0.8 ± 0.1 | 94.3 ± 2.5 | 97.1 ± 2.9 | 86.3 ± 3.4 |
| 10b | 4 | COOCH3 | 1.6 ± 0.7 | 1.4 ± 0.4 | 0.9 ± 0.2 | 92.6 ± 3.4 | 95.6 ± 3.7 | 85.8 ± 4.7 |
| 10c | 5 | COOC2H5 | 0.7 ± 0.2 | 1.5 ± 0.5 | 0.9 ± 0.2 | 95.4 ± 3.3 | 97.3 ± 2.9 | 87.0 ± 3.1 |
| 10d | 6 | COOCH3 | 0.9 ± 0.2 | 1.7 ± 0.3 | 0.8 ± 0.1 | 96.6 ± 3.2 | 96.7 ± 2.5 | 86.1 ± 2.3 |
| 11a | 3 | COOH | 0.6 ± 0.1 | 0.7 ± 0.2 | 0.9 ± 0.1 | 97.6 ± 2.8 | 97.1 ± 1.9 | 76.8 ± 2.6 |
| 11b | 4 | COOH | 1.1 ± 0.1 | 1.2 ± 0.1 | 0.7 ± 0.1 | 95.6 ± 2.4 | 97.2 ± 3.0 | 83.7 ± 3.5 |
| 11c | 5 | COOH | 0.8 ± 0.1 | 1.1 ± 0.1 | 0.7 ± 0.2 | 94.6 ± 2.8 | 95.4 ± 2.9 | 94.9 ± 0.6 |
| 11d | 6 | COOH | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.9 ± 0.2 | 97.6 ± 3.5 | 96.3 ± 1.9 | 97.8 ± 1.6 |
| 12a | 3 | CONHOH | 2.7 ± 0.1 | 1.3 ± 0.9 | 1.1 ± 0.3 | 85.7 ± 4.2 | 94.2 ± 1.1 | 95.3 ± 3.1 |
| 12b | 4 | CONHOH | 19.7 ± 2.1 | 2.8 ± 1.7 | 0.7 ± 0.6 | 54.1 ± 3.7 | 89.5 ± 4.2 | 89.3 ± 4.8 |
| 12c | 5 | CONHOH | 87.2 ± 2.0 | 81.5 ± 1.5 | 39.7 ± 4.1 | 44.1 ± 3.4 | 49.8 ± 3.9 | 44.2 ± 3.6 |
| 12d | 6 | CONHOH | 84.9 ± 3.5 | 77.1 ± 2.4 | 49.9 ± 3.5 | 38.6 ± 3.1 | 32.7 ± 3.4 | 39.1 ± 2.9 |
a
Data are presented as mean ± SD of at least three independent experiments performed in triplicate. Cells were treated with the indicated compounds at 1, 5, 10 μm for 24 h. The expression of GFP were evaluated by FACS.