Fig. 2. Antibody titer and its inhibition rate against AG evaluated by ELISAs. The closed squares and hatched bar graphs indicate absorbance at 405 nm evaluated by indirect ELISA and inhibition rate against AG (50 μg mL⁻¹). In both ELISAs, AG–OVA conjugates (1 μg mL⁻¹) were used as coated antigen, and 100 times diluted antisera with PBS-T were used as primary antibody.