Determination of AG in the members of plant family Gentianaceae by developed icELISA and HPLCa.
| Sample name | icELISA | HPLC | ||
|---|---|---|---|---|
| % (wt/dry wt) | CV (%) | % (wt/dry wt) | CV (%) | |
| S. japonica (powdered) | 1.26 × 10−1 ± 6.96 × 10−3 | 5.51 | 0.886 × 10−1 ± 2.26 × 10−3 | 2.55 |
| S. japonica (whole plant) | 1.95 × 10−1 ± 7.63 × 10−3 | 3.91 | 1.33 × 10−1 ± 3.58 × 10−3 | 2.70 |
| S. japonica (whole plant, Nagano, Japan) | 1.17 × 10−1 ± 7.11 × 10−3 | 6.07 | 0.811 × 10−1 ± 0.963 × 10−3 | 1.19 |
| G. lutea (powdered) | 3.91 × 10−2 ± 2.38 × 10−3 | 6.10 | Not quantitated | |
| G. scabra (powdered) | 2.61 × 10−3 ± 5.08 × 10−5 | 1.94 | Not detected (ND) | |
| G. scabra I (root) | 9.45 × 10−4 ± 7.53 × 10−5 | 7.97 | ND | |
| G. scabra II (root, Ibaraki, Japan) | 3.80 × 10−3 ± 1.19 × 10−4 | 3.13 | ND | |
a
CV represents coefficient of variation. All values were mean ± standard deviation (S.D.) from triplicate samples.