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. 2018 May 16;8(32):17860–17877. doi: 10.1039/c8ra00383a

Fig. 5. Evaluation of the osteogenic differentiation of hADSCs on CMC/nHA/GO substrates. (a) The protein expression of osteogenic markers of hADSCs incubated in PM or DM for 7 d; (b) BSP immunofluorescence staining of hADSCs on substrates incubated in PM for 7 d (scale bars: 50 μm); (c) positive cell ratios of BSP were determined by dividing the number of immune-positive cells to the number of nuclei stained with Hoechst (*P < 0.05, **P < 0.01, ***P < 0.001); (d) light microscopy and optical images of ALP staining of hADSCs on substrates incubated in PM or DM for 7 d and ARS staining of hADSCs on substrates incubated in PM or DM for 14 d (scale bars: 200 μm).

Fig. 5