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| Genes | Accession number | Forward (5′–3′) | Reverse (5′–3′) | Annealing temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|---|
| CK4 | XM_008256495.1 | CAACCTGAAGACCACCAAGA | CAGAGTCTGGCACTGCTTT | 60 | 100 |
| MUC5AC | XM_008253634.1 | TGATGACCAACCAGGTCATTT | GGGATGGTCACGTACATCTTG | 60 | 106 |
| GAPDH | NM_001082253 | GGTCGGAGTGAACGGATTT | TGTAGTGGAGGTCAATGAATGG | 60 | 113 |
| IL-6 | NM_001082064.2 | TGTACGATCACTGAACTGCA | GAAGTCAGTTATATCCTGGC | 60 | 291 |
