Fig. 1. MiR-320d is down-regulated in breast cancer and is associated with poor survival. (A) The expression of miR-320d in tumor tissues and adjacent normal tissues was analyzed by qRT-PCR, n = 34. (B) The expression of miR-320d in MCF-10A, MDA-MB-468, SKBR3, MCF-7, MDA-MB-453, T47D and MDA-MB-231 cells was analyzed by qRT-PCR. (C) Kaplan–Meier survival analysis of breast cancer patients. Low expression (n = 20) and high expression (n = 14) of miR-320d were defined by using the median values of miR-320d expression levels in the 34 breast cancer tissues. All the experiments were repeated three times. *P < 0.05 versus normal tissues or MCF-10A cells.