Fig. 8. Compound F779-0434 inhibited Rad52–ssDNA association. (A). Purified human RAD52 protein was analyzed by SDS-PAGE. (B). Western blot analysis of RAD52 expressing plasmid-transformed E. coli lysate and purified RAD52 protein with anti-Histag antibody, lane 1, uninduced total E. coli lyaste; Lane 2, 0.25 mM IPTG induced E. coli lysate; lane 3, purified Rad52 protein; (C). Schematic illustration of the pull-down assay performed to analyze RAD52–ssDNA interaction. (D). A representative SDS-PAGE of the pull-down assay result; (E). Quantification of RAD52 pulled down by ssDNA. The experiments were repeated at least three times. Error bars indicate standard error of the mean (SEM).