Lower limit of detection (LLOD), LLOQ, linear range, regression coefficient and stability for methodology development^a.

Steroid hormones	Derivatization reagent	LLOD (pg mL−1)	LLOQ (pg mL−1)	Linear range (pg mL−1)	Regression coefficient (R2)	Stability within 24 h RSD (%)
17αOH-PROG	DMBA	2	5	5–20 000	0.7944	1.3
E1	DMBA	5	10	10–20 000	0.9822	3.2
2-Methoxyestrone	DMBA	2	20	20–20 000	0.9881	6.2
Tetrahydrocortisol	DMBA	5	50	50–20 000	0.9942	3.1
Corticosterone	DMBA	20	50	50–20 000	0.9820	12
E2	DMBA	10	20	20–20 000	0.9941	15
Pregnandiol	DMBA	20	50	50–20 000	0.9911	3.4
19-Hydroxyandrostenedione	DMBA	50	200	100–40 000	0.9915	ND
Cortol	DMBA	100	200	200–40 000	0.9709	1.0
Androsterone	GP	<2	5	5–20 000	0.9999	2.5
11-Deoxycorticosterone	GP	2	5	5–20 000	0.9979	5.3
THB	GP	2	5	5–20 000	0.9991	8.8
5β-Pregnane-3,20-dione	GP	2	5	5–20 000	0.9984	1.4
PREG	GP	2	5	5–20 000	0.9981	ND
E3	GP	5	10	10–20 000	0.9997	7.2
TES	GP	2	10	10–20 000	0.9994	6.9
17αOH-PREG	GP	5	10	10–20 000	0.9993	1.5
DHEA	GP	5	20	20–20 000	0.9999	12
Cortisone	GP	20	50	50–40 000	0.9909	2.0
11β,17α,21-Trihydroxy-5β-pregnane-3,20-dione	GP	500	1000	1000–40 000	0.9967	ND
PROG	GP	2500	5000	5000–40 000	0.9986	ND

^aND: not detected.