FIG. 1.

SSCP analysis of four genomic loci (indicated at the left) for P. carinii f. sp. hominis isolates. Mixture represents either wild-type and double mutation (A/T-55 and S/P-57) or single A-55 mutation and double mutation (A-55 and S/P-57). The DHPS genotype is indicated at the top. Patient numbers (1 to 22) are shown under the DHPS genotypes. Below each lane is the genotype for each locus. The genotypes for 26S rRNA and tubulin were named based on SSCP patterns alone, and the genotypes for ITS1 and mt26S were determined by SSCP analysis combined with DNA sequencing. Undetermined genotypes are indicated by “★.” The genotypes for ITS1 correspond to those described previously (9), with the variable numbers of T at positions 62 to 71 given in superscript (“−1” and “+1” represent 9 and 11 T's, respectively, compared to the commonly seen 10 T's). For mt26S, all five genotypes contain a G-to-A change at position 288 compared to the published sequenced (14), with an additional C-to-A change at position 85, a C-to-T change at position 248, a C-to-T change at position 85, or a C-to-T change at position 80 in types 2, 3, 4, and 5, respectively.