Fig. 5. (a) Growth of PANC-1 cells in uncoated (PUN) and FN coated (PFN) PU scaffolds for 29 days. Data are presented as mean ± S.D. (N = 2, n = 3). Statistical differences are marked by asterisks (*p < 0.05; **p < 0.01). N = number of independent experiments; n = number replicates. (b and c) Visualization of PANC-1 cells in uncoated (PUN) and FN coated (PFN) scaffolds respectively with fluorescence Live (green, Calcein AM) and Dead (red, Ethidium homodimer-1) viability assay at day 29 of culture.