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. 2018 Jun 7;8(37):20928–20940. doi: 10.1039/c8ra02633e

Primary and isotype antibody concentrations for the immunofluorescence (IF) assay^a.

	Primary	Isotype
Fig. 4a	11.17 μg mL⁻¹ rat anti human Ki-67	11.17 μg mL⁻¹ rat IgG1^b
	16.67 μg mL⁻¹ mouse anti human HIF-1α	16.67 μg mL⁻¹ mouse IgG2b
	8.34 μg mL⁻¹ rabbit anti human collagen-I	8.34 μg mL⁻¹ rabbit IgG
Fig. 4b and c	8.00 μg mL⁻¹ rabbit anti human LC3 A/B	8.00 μg mL⁻¹ rabbit IgG
Fig. 4b and c	16.67 μg mL⁻¹ mouse anti human HIF-1α	16.67 μg mL⁻¹ mouse IgG2b
Fig. 6	16.67 μg mL⁻¹ mouse anti human HIF-1α	16.67 μg mL⁻¹ mouse IgG2b
Fig. 6	8.34 μg mL⁻¹ rabbit anti human collagen-I	8.34 μg mL⁻¹ rabbit IgG
Fig. 7	11.17 μg mL⁻¹ rat anti human Ki-67	11.17 μg mL⁻¹ rat IgG1^b
	16.67 μg mL⁻¹ mouse anti human HIF-1α	16.67 μg mL⁻¹ mouse IgG2b
	8.34 μg mL⁻¹ rabbit anti human collagen-I	8.34 μg mL⁻¹ rabbit IgG

^a

All products were obtained from Abcam, UK.

^b

IgG: immunoglobulin G.