Fig. 9. Illustration of the methodology flow sheet. Functional and structural characterization of PCNXL2 was achieved by performing 10 steps. The sequence was retrieved from NCBI. Physiochemical properties and conserved domains were predicted using the Pfam and Protpram tools. SOUSI server was used to identify the location and nature of the protein. The interactions of PCNXL2 with other proteins were investigated by string analysis. CYSPRED, CYSCON and DIANA were used to estimate disulphide bonding patterns within the protein. The structure was predicted using Raptor X and ITASSER. miRTarBase was used to identify miRNAs targeting PCNXL2 in different cancers. COACH was used to identify protein ligand binding sites. Finally, the pathway was designed by reviewing literature studies and KEGG pathways.