Fig. 5. NIH/3T3 cellular elongation and orientation on the aligned micelles. (a) Representative confocal image of cell and fitting ellipse used to measure elongation (ratio of major axis/minor axis) and orientation (angle θ). (b) Cellular aspect ratio as a function of micelle covering percentage. (c) Distribution of NIH/3T3 cells orientation angle (θ) on glass substrates with various area percentages of micelle coverages: 0%, 4.4%, 9.5%, 20.0% and 31.4%. The blue lines are the corresponding Gaussian fitting curves.