Fig. 7. NIH/3T3 nuclear elongation and orientation on the aligned micelles. (a) Representative confocal image of nucleus and fitting ellipse used to measure elongation (ratio of long axis/short axis) and orientation (angle θ). (b) Nuclear aspect ratio as a function of micelle covering percentage. (c) Distribution of NIH/3T3 nuclei orientation on glass substrates with various area percentages of micelle coverages: 0%, 4.4%, 9.5%, 20.0% and 31.4%.