Figure 1.

Crystal structure of poxvirus decapping enzyme D9. (A) Block diagram of VACV-WR D9 domains (above) and crystal structure of VACV-WR D9 bound to m⁷GDP product, PDB 7SEZ (below). D9 catalytic Nudix domain is teal, insertion domain is grey, Nudix helix is red, m⁷GDP is represented as spheres. (B) Block diagram of S. pombe Dcp2 domains (above) and crystal structure of the m⁷GDP product bound S. pombe Dcp1/Dcp2 decapping complex, PDB 5N2V (below) (Wurm et al., 2017). NRD is the N-terminal regulatory domain, CD is the catalytic domain, IDR is the intrinsically disordered region. Dcp1 is yellow, Dcp2 N-terminal regulatory domain (NRD) is purple, Dcp2 catalytic Nudix domain (CD) is green, Nudix helix is red, m⁷GDP is represented as spheres. (C) Topology maps of D9 (left) and Dcp2 (right). Coloring is the same as in (A) and (B).