Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and virus strains | ||
| E. coli BL21 (DE3) | New England Biolabs | Cat# C2527H |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| L-(+)-Selenomethionine | Fisher Scientific | Cat# AC259960025 |
| α–32P-GTP | PerkinElmer | Cat# BLU006H500UC |
| γ–32P-ATP | PerkinElmer | Cat# NEG502A250UC |
| Cap analogs (m7GDP and trinucleotide substrate) | Jacek Jemielity Lab | N/A |
| T4 PNK | New England Biolabs | Cat# M0201S |
| Critical commercial assays | ||
| HiTrap Heparin HP Column | GE Healthcare | Cat# 95056–036 |
| GE Superdex 75 16/60 Gel Filtration Column | Cytiva | Cat# 28989333 |
| Deposited data | ||
| D9 wild-type bound to m7GDP product | This paper | PDB: 7SEZ |
| D9 wild-type bound to trinucleotide substrate | This paper | PDB: 7SF0 |
| S. pombe Dcp1/Dcp2 decapping complex | (Wurm et al., 2017) | PDB: 5N2V |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| RNA sequences used for binding and decapping assays, see Table S2 | This paper | N/A |
| Sense T7 transcription template: TAATACGACTCACTATAGGG | Integrated DNA Technologies, This paper | N/A |
| Anti-sense 29mer 5’ overhang transcription template: mCmAGCACGACATAACACTAACCCTATAGTGAGTCGTATTA | Integrated DNA Technologies, This paper | N/A |
| Anti-sense 29mer blunt end transcription template: mGmGGCAGATTACAGCACGACATAACACTAACCCTATAGTGAGTCGTATTA | Integrated DNA Technologies, This paper | N/A |
| Anti-sense 29mer 3’ overhang transcription template: mGmGTAGTTATTGGGCAGATTACAGCACGACATAACACTAACCCTATAGTGAGTCGTATTA | Integrated DNA Technologies, This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pET28b-D9, various mutants | This paper | N/A |
| Software and algorithms | ||
| XDS | (Kabsch and IUCr, 2010) | https://xds.mr.mpg.de/ |
| Pointless, Aimless (CCP4 packages) | (Winn et al., 2011) | https://www.ccp4.ac.uk/ |
| Phenix 1.9–1692 | (Adams et al., 2010) | https://phenix-online.org/ |
| Coot 0.9.6 | (Emsley et al., 2010) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Pymol 2.4.2 | Schrödinger | https://pymol.org/2/ |
| Igor Pro 6.3.7 | WaveMetrics | https://www.wavemetrics.com/products/igorpro |
| Quantity One 4.6.6 | Bio-Rad | https://www.bio-rad.com/en-us/product/quantity-one-1-d-analysis-software?ID=1de9eb3a-1eb5-4edb-82d2-68b91bf360fb |
| Jalview 2.10.5 | (Waterhouse et al., 2009) | http://www.jalview.org/getdown/release/ |
| Other | ||