FIGURE 5.

Cell velocity is increased in SMA patient iPSC derived microglia compared to control. Velocity and persistence of control (n = 5; minimum of 14 cells per group) and SMA patient iPSC derived microglia (n = 5; minimum of 14 cells per group) was performed using video analysis over 16 h, with images taken every 10 min. (a) Velocity was significantly increased, **p = .0061. SMN levels were restored in SMA microglia (n = 3; minimum of 50 cells per group) and velocity and analyzed. Transiently expressing SMN restored the cell velocity of SMA microglia to control cell levels. ****p = <.0001. (b) No change in persistence was detected in SMA versus control microglia. Restoring SMN levels did not alter persistence. All groups were analyzed by unpaired t‐test. Values represent mean ± SEM. (c) Representative photomicrographs depicting unaffected and SMA iPSC‐derived microglia plated in a monolayer following a scratch wound. A single wound was administered, and cell chamber was imaged every 10 min for 16 h and (d) total area occupied by migrating cells and, (e) total distance covered over time (rate) was quantified using ImageJ. (area covered **p = .0024; rate **p = .0051). Significance was determined by one‐way ANOVA followed by post hoc Tukey's test for multiple comparisons. Values represent mean ± SEM